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Title   대장암에서 발견된 염색체이상과 암유전자의 발현 ( Chromosomal Abnormalities Found in the large Bowel Cancer and Oncogene Expression )
Publicationinfo   1993 Jan; 025(02): 182-196.
Key_word   Oncogene, DNA in situ hybridization, Chromosomal abnormalities, Marker chromosome, Antioncogene
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Abstract   To investigate common specific chromosome abnormalities and related oncogene in large bowel cancer, four primary large bowel cancer and cell line(CoLo 320 DM) were cultured and karyotyped with G-banding. DNA in situ hybridization and Sourthern blot were also performed on the same materials with erbB-2, fos, K-ras and H-ras oncogenes. Hyperdiploidy in primary cancers and tetraploid in cancer cell line were the common numerical chromosome abnormalities. The common structural abnormalities in primary cancers were translocation on chromosome 4pter and 5pter of which derivatives region were unknown and deletion on chromosomes 1p22, 3q11 and 6q32 on the other hand, t(4pter;?) and del(3ql 1, 6q22) were found in cancer cell line. The genes which located in 4pter and 3q11 which were common finding in primary cancers and cell line were important for futher study in oncogenesis of large bower cancer. ErbB-2 was translocated from 17q21 to 2p and 4p and, fos was translocated from 14q21 to 4q and 1 1q. K-ras and H-ras was the same bands with normal mucosa cells of large boweL It was not directly corelated that the chromosomal regions which were translocated of erbB-2 and fos were found chromosome abnormalities by cytogenetic analysis.
저 자   양동휘(Dong Wheuy Yang),손수상(Soo Sang Sohn),강중신(Joong Shin Kang),장성익(Sung Ik Chang)